Micropropagation and in Vitro Flowering of an Ornamental Aquarium Plant Lindernia antipoda L. (Alston)  

Jabir T. , Sheeja George , Anjara Raj , Sree Lakshmi S. , Aneykutty Joseph
Department of Marine Biology, Microbiology & Biochemistry, School of Marine Science, Cochin University of Science and Technology, Kerala, India
Author    Correspondence author
International Journal of Aquaculture, 2016, Vol. 6, No. 8   doi: 10.5376/ija.2016.06.0008
Received: 29 Feb., 2016    Accepted: 12 Apr., 2016    Published: 23 Sep., 2016
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This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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Jabir T., Sheeja George., Anjana Raj., Sree Lakshmi S., Aneykutty Joseph., 2016, Micropropagation and in vitro flowering of an ornamental aquarium plant Lindernia antipoda (L.) Alston, 6(8): 1-10 (doi: 10.5376/ija.2016.06.0008)

Lindernia antipoda L. (Alston) is one of the valuable aquatic ornamental plants which have a bright potential in the aquarium trade. In the present study a protocol for rapid shoot multiplication and in vitro flowering from axillary bud was developed. Half MS media with 1 mg/L benzylaminopurine (BAP) was the found to be the best medium for culture initiation and establishment. The effective medium for shoot multiplication was MS+1 mg/L BAP+0.2 mg/L NAA. In this media, 85% of the cultures exhibited multiple shoot proliferation. The highest shoot proliferation and shoot length were also acquired in this media. Plant showed highest in vitro flowering in MS medium supplemented 1 mg/L BAP and 0.2 mg/L α-Naphthalene acetic acid (NAA). All concentrations of NAA induced rooting. Hardening of in vitro cultured plantlets were attained through polythene propagator system and 90% of the plants survived, and it was successfully transformed in to an aquarium.
Lindernia antipoda; Aquarium plant; Multiple shoot proliferation; In vitro flowering
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