Assessment of Genetic Variability of Prawn (Macrobrachium rosenbergii) Post Larvae (PL) from the Broods Stocked under Different Sex Ratios  

Shahinur Sheikh Islam1 , Md. Saifuddin Shah2 , Md. Lifat Rahi2
1. Research Centre for Experimental Marine Biology and Biotechnology, University of the Basque Country, Spain
2. Fisheries and Marine Resource Technology Discipline and Vice-chancellor, Khulna University, Khulna-9208, Bangladesh
Author    Correspondence author
International Journal of Aquaculture, 2014, Vol. 4, No. 9   doi: 10.5376/ija.2014.04.0009
Received: 21 Jan., 2014    Accepted: 27 Feb., 2014    Published: 24 Mar., 2014
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This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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Islam et al., 2014, Assessment of Genetic Variability of Prawn (Macrobrachium rosenbergii) Post Larvae (PL) from the Broods Stocked under Different Sex Ratios, International Journal of Aquaculture, Vol.4, No.09: 55-63 (doi: 10.5376/ija.2014.04.0009)

The giant freshwater prawn (Macrobrachium rosenbergii) culture in Bangladesh is now at stake due to slower growth performance. The aim of the present study was to assay the impact of sex ratio on the genetic variability of larvae produced from the broods stocked under different sex ratios by analyzing Randomly Amplified Polymorphic DNA (RAPD) of M. rosenbergii. Broods were stocked under 3 different sex ratios viz., 1♂: 1♀, 1♂: 2♀ and 2♂: 1♀. A control population was also kept that involved the larvae production from the broods of natural source. The RAPD analysis generated 88 distinct bands of which 36 (41%) bands were polymorphic. The percentages of polymorphic loci were found to be 32%, 48%, 24% and 52% for the sex ratios 1♂: 2♀, 1♂: 1♀, 2♂: 1♀ and Control respectively. The pair wise genetic distances were 0.010, 0.007, 0.021, 0.016, 0.026 and 0.008 for 1♂: 1♀ vs. 1♂: 2♀, 1♂: 1♀ vs. Control, 1♂: 1♀ vs. 2♂: 1♀, 1♂: 2♀ vs. Control, 2♂: 1♀ vs. Control and 1♂: 2♀ vs. 2♂: 1♀ respectively. Overall gene diversity for all loci was 0.10. The unweighted pair group method of arithmetic mean (UPGMA) dendrogram based on Nei’s genetic distances clearly showed the severance of four different experimental groups into two clusters: 1♂: 1♀ and control in one and 1♂: 2♀ and 2♂: 1♀ in the other cluster. Results of the present study revealed that 1♂: 1♀ sex ratio is appropriate to establish the brood banks and for maintaining genetic variation in the offspring.
Giant freshwater prawn; Genetic variability; Sex ratios; Polymorphism
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